This invention relates to a composition of acylated oligopeptide
and aqueous fulvic acid extract product for treatment of fine lines and
wrinkles in facial skin by improving fibroblast matrix and a method of
using the composition. Preferably, the acyl oligopeptide is a palmitoyl
hexapeptide based on the amino acids phenylalanine, alanine, leucine and
lysine. The fulvic acid is the water-soluble fraction of acidified humus,
obtained from naturally occurring peat. The composition may contain dimethylisosorbide
or ethoxydiglycol as solubilizing and penetration enhancers for the acyl-modified
oligopeptide and other preferred skin beneficial ingredients.
BACKGROUND OF THE INVENTION
U.S. Pat. No. 6,875,744 B2 specifies short bioactive peptides
that are primarily made from phenylalanine, alanine, leucine and lysine.
Such peptides are water soluble or miscible and have been reported to offer
a low in-vitro minimum inhibitory concentration (MIC) against P. acnes
. Benefits for hydrophobic acylated peptides of similar sequences have
been reported include increasing cell density of fibroblast cells in the
Cyquant cell proliferation assay (Molecular probes, C-7026) due to boosting
the immune system by acting like a host defense peptide. Host defense peptides
boost the innate immune system and have been shown to have a number of
immunomodulatory functions including altering host gene expression, acting
as chemokines and/or inducing chemokine production, inhibiting lipopolysaccharide
induced pro-inflammatory cytokine production, promoting wound healing,
and modulating the responses of dendritic cells and cells of the adaptive
immune response. The boosting of such aspects of healthy skin are the same
as are required for younger and less-wrinkled skin.
It is not generally recommended that such peptides be
stored in solution. However, the shelf life of peptides is extended for
sequences not containing Cysteine, Methionine, Tryptophan, Asparagine and
Glutamine.
Peptides are not very useful if they are insoluble in
the aqueous buffers required for testing in bioassay systems. Peptides
can be made more lipophilic with C2-C22 alkyl esters or amides on the terminus
to modify the interaction of the peptide with skin lipids but this modification
drastically changes the solubility and makes peptides even more difficult
to deliver in a stable aqueous cosmetic vehicle. Many biochemists use dimethylformamide
or dimethylsulfoxide to help dissolve peptides for aqueous bioassay. Both
of these solvents are not considered acceptable for cosmetic use. Solvents
like ethoxydiglycol or dimethylisosorbide are useful cosmetic solvents
for enhancing skin activity and can help solubilize the lipophilic peptide
in an oil phase. Formulation of excess lipophilic solvent can remove surface
lipids and leave the skin feeling dry or brittle. U.S. Pat. No. 4,814,173
teaches the combination of dimethylisosorbide, peptide and silicone elastomers
as part of a preferred transdermal matrix system.
Fulvic acid is the water-soluble fraction of acidified
humus. Humus is the naturally occurring decomposition product of vegetation
as found in peat sources. Fulvic acid is thought to aid transport of minerals
to cells. US application 20060257437 (Ingman) used a combination of water,
silica, and optionally silver ions, in a cosmetic containing fulvic acid
for concealing wrinkles and improving skin condition, but did not employ
peptides in the cosmetic specification.
Commonly assigned application Ser. No. 11/654,406 was
filed on 17 Jan. 2007, claims the benefit of the 18 Jan. 2006 filing date
of Provisional Application Ser. No. 60/760,034, and is incorporated-by-reference
in its entirety. Ser. No. 11/654,406 discloses that specific short chain
acylated peptides in combination with lycium barbarum (goji berry) glyco-conjugates
lead to improved dermal fibroblast activity, resulting in anti-wrinkle
benefits in cosmetic applications.
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